Cytokeratin 12 Antibody (2M1K10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33197
Key Product Details
Species Reactivity
Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 2M1K10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 250-494 of human Cytokeratin 12 (KRT12) (NP_000214.1).
Sequence:
EMQIESLNEELAYMKKNHEDELQSFRVGGPGEVSVEMDAAPGVDLTRLLNDMRAQYETIAEQNRKDAEAWFIEKSGELRKEISTNTEQLQSSKSEVTDLRRAFQNLEIELQSQLAMKKSLEDSLAEAEGDYCAQLSQVQQLISNLEAQLLQVRADAERQNVDHQRLLNVKARLELEIETYRRLLDGEAQGDGLEESLFVTDSKSQAQSTDSSKDPTKTRKIKTVVQEMVNGEVVSSQVQEIEELM
Sequence:
EMQIESLNEELAYMKKNHEDELQSFRVGGPGEVSVEMDAAPGVDLTRLLNDMRAQYETIAEQNRKDAEAWFIEKSGELRKEISTNTEQLQSSKSEVTDLRRAFQNLEIELQSQLAMKKSLEDSLAEAEGDYCAQLSQVQQLISNLEAQLLQVRADAERQNVDHQRLLNVKARLELEIETYRRLLDGEAQGDGLEESLFVTDSKSQAQSTDSSKDPTKTRKIKTVVQEMVNGEVVSSQVQEIEELM
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Cytokeratin 12 Antibody (2M1K10) (NBP3-33197) is a monoclonal antibody validated for use in IHC, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Cytokeratin 12 Antibody (2M1K10)
Immunohistochemistry: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] -
Immunohistochemistry: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Immunohistochemistry analysis of paraffin-embedded Rat cornea using Cytokeratin 12(KRT12) (KRT12) Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] -
Immunohistochemistry: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Immunohistochemistry analysis of paraffin-embedded Mouse cornea using Cytokeratin 12(KRT12) (KRT12) Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] -
Immunocytochemistry/ Immunofluorescence: Cytokeratin 12 Antibody (2M1K10) [NBP3-33197] - Confocal imaging of paraffin-embedded Rat eyeball tissue using Cytokeratin 12 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.Applications for Cytokeratin 12 Antibody (2M1K10)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytokeratin 12
Alternate Names
CK12, K12, keratin 12, keratin, type I cytoskeletal 12
Gene Symbol
KRT12
Additional Cytokeratin 12 Products
Product Documents for Cytokeratin 12 Antibody (2M1K10)
Product Specific Notices for Cytokeratin 12 Antibody (2M1K10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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