Cystatin B/Stefin B Antibody (24GB3050)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50683

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NBP3-50683-100ul
NBP3-50683-20ul
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Key Product Details

Species Reactivity

Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB3050

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cystatin B Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Cystatin B/Stefin B (Uniprot: P04080)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cystatin B/Stefin B Antibody (24GB3050)

Cystatin B/Stefin B Antibody (24GB3050)

Immunocytochemistry/ Immunofluorescence: Cystatin B/Stefin B Antibody (24GB3050)[NBP3-50683] -

Immunocytochemical staining of H9C2 cells with Cystatin B/Stefin B antibody (1:1,000). Nuclei were stained blue with DAPI; Cystatin B/Stefin B was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
Cystatin B/Stefin B Antibody (24GB3050)

Flow Cytometry: Cystatin B/Stefin B Antibody (24GB3050)[NBP3-50683] -

Flow cytometric analysis of Cystatin B/Stefin Bexpression in H9c2 cells using Cystatin B/Stefin B antibody (1:2,000). Green, isotype control; red, Cystatin B.
Cystatin B/Stefin B Antibody (24GB3050)

Western Blot: Cystatin B/Stefin B Antibody (24GB3050)[NBP3-50683] -

Western blotting analysis using Cystatin B/Stefin B antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Cystatin B/Stefin B Antibody (24GB3050)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cystatin B

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1).

Alternate Names

CSTB, Stefin B

Gene Symbol

CSTB

Additional Cystatin B Products

Product Documents for Cystatin B/Stefin B Antibody (24GB3050)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cystatin B/Stefin B Antibody (24GB3050)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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