Cyclin E2 Antibody (0Y7G5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16806
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0Y7G5 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Cyclin E2 (O96020). HQYEIRNCWPPVLSGGISPCIIIETPHKEIGTSDFSRFTNYRFKNLFINPSPLPDLSWGCSKEVWLNMLKKESRYVHDKHFEVLHSDLEPQMRSILLDWLL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Cyclin E2 Antibody (0Y7G5) (NBP3-16806) is a recombinant monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Cyclin E2 Antibody (0Y7G5)
![Western Blot: Cyclin E2 Antibody (0Y7G5) [NBP3-16806]](https://resources.bio-techne.com/images/products/Cyclin-E2-Antibody-0Y7G5-Western-Blot-NBP3-16806-img0001.jpg "Western Blot: Cyclin E2 Antibody (0Y7G5) [NBP3-16806]")
Western Blot: Cyclin E2 Antibody (0Y7G5) [NBP3-16806]
Western Blot: Cyclin E2 Antibody (0Y7G5) [NBP3-16806] - Western blot analysis of extracts of various cell lines, using Cyclin E2 Rabbit mAb (NBP3-16806) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Applications for Cyclin E2 Antibody (0Y7G5)
Application
Recommended Usage
Western Blot
1:100 - 1:500
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cyclin E2
Cyclin E2 (CCNE2) is an approximately 45-50 kDa protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression and the initiation of DNA replication. Cyclin E2 is required for G1/S phase progression and cell cycle reentry from G0 phase. Cyclin E2 most highly expressed in testis, thymus, and brain and is frequently upregulated in cancer. Cyclin E2 contains two cyclin box folds (aa 141-231 and aa 241-361). An alternate splice form lacks 45 amino acids within the first cyclin box fold.
Alternate Names
CCNE2, CYCE2
Gene Symbol
CCNE2
Additional Cyclin E2 Products
Product Documents for Cyclin E2 Antibody (0Y7G5)
Product Specific Notices for Cyclin E2 Antibody (0Y7G5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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