Cyclin D3 Antibody (4C5C8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16309
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4C5C8 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 202-292 of human Cyclin D3 (P30281). MIATGSIGAAVQGLGACSMSGDELTELLAGITGTEVDCLRACQEQIEAALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Cyclin D3 Antibody (4C5C8) (NBP3-16309) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Cyclin D3 Antibody (4C5C8)
![Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309]](https://resources.bio-techne.com/images/products/Cyclin-D3-Antibody-4C5C8-Western-Blot-NBP3-16309-img0001.jpg "Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309]")
Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309]
Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of various cell lines, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.![Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309]](https://resources.bio-techne.com/images/products/Cyclin-D3-Antibody-4C5C8-Western-Blot-NBP3-16309-img0002.jpg "Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309]")
Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309]
Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of Rat lung, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Applications for Cyclin D3 Antibody (4C5C8)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cyclin D3
During the G1 phase of the cell cycle, a cell must choose between rest and cell division. A small subfamily of cyclin family molecules, termed cyclin D proteins (D1, D2, D3), promotes commitment to mitosis. They accomplish this by interacting with cyclin-dependent kinases (cdk4 and cdk6) to generate Ser/Thr kinase complexes that remove blocks on DNA synthesis.
Alternate Names
CCND3
Gene Symbol
CCND3
Additional Cyclin D3 Products
Product Documents for Cyclin D3 Antibody (4C5C8)
Product Specific Notices for Cyclin D3 Antibody (4C5C8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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