Cyclin B1 Antibody (24GB4300)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51628
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB4300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Cyclin B1 (Uniprot: P14635)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cyclin B1 Antibody (24GB4300)
Immunocytochemistry/ Immunofluorescence: Cyclin B1 Antibody (24GB4300)[NBP3-51628] -
Immunocytochemical staining of Hela cells with Cyclin B1 antibody (1:1,000) . Nuclei were stained blue with DAPI;Cyclin B1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium.Scale bar, 20 um.
Flow Cytometry: Cyclin B1 Antibody (24GB4300)[NBP3-51628] -
Flow cytometric analysis of Cyclin B1expression in HeLa cells using Cyclin B1 antibody (1:2,000). Green, isotype control; red, Cyclin B1.
Western Blot: Cyclin B1 Antibody (24GB4300)[NBP3-51628] -
Western blotting analysis using Cyclin B1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.Applications for Cyclin B1 Antibody (24GB4300)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cyclin B1
Alternate Names
CCNB1
Gene Symbol
CCNB1
Additional Cyclin B1 Products
Product Documents for Cyclin B1 Antibody (24GB4300)
Product Specific Notices for Cyclin B1 Antibody (24GB4300)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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