Cullin 4a Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2267
![Western Blot: Cullin 4a Antibody [NB100-2267]](https://resources.bio-techne.com/images/products/Cullin-4a-Antibody-Western-Blot-NB100-2267-img0002.jpg "Western Blot: Cullin 4a Antibody [NB100-2267]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 700 and the C-terminus (residue 759) of human Cullin 4A using the numbering given in entry NP_001008895.1 (GeneID 8451).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cullin 4a Antibody - BSA Free
Western Blot: Cullin 4a Antibody [NB100-2267]
Western Blot: Cullin 4a Antibody [NB100-2267] - Detection of Human and Mouse Cul4a by WB (h&m) and Immunoprecipitation (h). Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Cul4a antibody NB100-2267 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). Cul4a was also immunoprecipitated by rabbit anti-Cul4a antibody NB100-2266.![Immunohistochemistry-Paraffin: Cullin 4a Antibody [NB100-2267]](https://resources.bio-techne.com/images/products/Cullin-4a-Antibody-Immunohistochemistry-Paraffin-NB100-2267-img0007.jpg "Immunohistochemistry-Paraffin: Cullin 4a Antibody [NB100-2267]")
Immunohistochemistry-Paraffin: Cullin 4a Antibody [NB100-2267]
Immunohistochemistry-Paraffin: Cullin 4a Antibody [NB100-2267] - Section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Cul4a used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for Cullin 4a Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CUL4A
Long Name
Cullin 4a
Alternate Names
Cullin 4a
Gene Symbol
CUL4A
UniProt
Additional CUL4A Products
Product Documents for Cullin 4a Antibody - BSA Free
Product Specific Notices for Cullin 4a Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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