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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for CrkRS Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human cell division cycle 2 (CDC2)-related kinase arginine/serine-rich using the numbering given in entry NP_057591.2 (GeneID 51755).

Predicted Species

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CrkRS Antibody

Western Blot: CrkRS Antibody [NB100-87011] - Detection of Human and Mouse CrkRS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: CrkRS Antibody [NB100-87011] - Detection of human CrkRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for IP at 6 ug per reaction. For blotting immunoprecipitated CrkRS, NB100-87011 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for CrkRS Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in WB reported in scientific literature ( PMID 24478330).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 6 publications using NB100-87011 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CrkRS

CrkRS (Cdc2-related kinase, arginine/serine-rich) is a Cdc-2 related kinase that contains an arginine- and serine-rich (SR) domain and has recently been reported to interact with cyclin L1 and L2. Since it has been discovered that CrkRs pairs with a cyclin partner, it has also been given the name CDK12. CrkRs localizes to nuclear speckles and immunoprecipitates with RNA polymerse II and the SF2/ASF splicing factor and is proposed to play a role in the regulation of alternative splicing. Alternate names for CrkRS include cell division cycle 2-related protein kinase 7, CDC2-related protein kinase 7, CRKRS, CRK7, and KIAA0904.

Alternate Names

Cdc2-related kinase, arginine/serine-richCrkRS, Cell division cycle 2-related protein kinase 7, Cell division protein kinase 12, CRK7CDC2-related protein kinase 7, CRKR, CRKRS, cyclin-dependent kinase 12, EC 2.7.11, EC 2.7.11.23, hCDK12, KIAA0904EC 2.7.11.22

Entrez Gene IDs

51755 (Human)

Gene Symbol

CDK12

UniProt

Product Documents for CrkRS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CrkRS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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