Creatine Kinase BB Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00288
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human Creatine Kinase BB (Accession#: P12277; Pro2-Lys381)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Creatine Kinase BB Antibody - Azide and BSA Free
Immunoprecipitation: Creatine Kinase BB Antibody [NBP3-00288] -
Immunoprecipitation: Creatine Kinase BB Antibody [NBP3-00288] - CKB was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate 2 uL NBP3-00288 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00288, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size: 43 kDa
Western Blot: Creatine Kinase BB Antibody [NBP3-00288] -
Western Blot: Creatine Kinase BB Antibody [NBP3-00288] - NBP3-00288 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDaApplications for Creatine Kinase BB Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Creatine Kinase BB
Alternate Names
B-CK, CKB
Gene Symbol
CKB
Additional Creatine Kinase BB Products
Product Documents for Creatine Kinase BB Antibody - Azide and BSA Free
Product Specific Notices for Creatine Kinase BB Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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