CRABP2 Antibody (004) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89836
Recombinant Monoclonal Antibody
![Western Blot: CRABP2 Antibody (004) [NBP2-89836]](https://resources.bio-techne.com/images/products/CRABP2-Antibody-004-Western-Blot-NBP2-89836-img0002.jpg "Western Blot: CRABP2 Antibody (004) [NBP2-89836]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 004 expressed in HEK293
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human CRABP2 (Accession#: NP_001869.1; Pro2-Glu138).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CRABP2 Antibody (004) - Azide and BSA Free
Western Blot: CRABP2 Antibody (004) [NBP2-89836]
Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa![Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836]](https://resources.bio-techne.com/images/products/CRABP2-Antibody-004-Flow-Cytometry-NBP2-89836-img0004.jpg "Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836]")
Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836]
Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836] - Analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated , stained with purified anti-Human CRABP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.![Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836]](https://resources.bio-techne.com/images/products/CRABP2-Antibody-004-Immunoprecipitation-NBP2-89836-img0003.jpg "Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836]")
Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836]
Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate0.5 uL anti-CRABP2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CRABP2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 16 kDa Observed band size: 16 kDaApplications for CRABP2 Antibody (004) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100-1:500
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunoprecipitation
0.1-0.5 ul/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CRABP2
Long Name
Cellular Retinoic Acid Binding Protein 2
Alternate Names
CRABP-II, RBP6
Gene Symbol
CRABP2
Additional CRABP2 Products
Product Documents for CRABP2 Antibody (004) - Azide and BSA Free
Product Specific Notices for CRABP2 Antibody (004) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...