COX IV Isoform 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44068

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NBP1-44068
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all COX4-I2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RWDYEKKQWKK corresponding to C-Terminus according to NP_115998.2.

Epitope

C Terminus

Specificity

This antibody is expected to recognize COX4I1 (GeneID:1327; MW: 19.6kDa) & COX4I2 (GeneID:84701, MW:19.6kDa).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for COX IV Isoform 2 Antibody

Western Blot: COX IV Isoform 2 Antibody [NBP1-44068]

Western Blot: COX IV Isoform 2 Antibody [NBP1-44068]

Western Blot: COX IV Isoform 2 Antibody [NBP1-44068] - Analysis of COX IV Isoform 2 in Human Skeletal Muscle lysate (35ug protein in RIPA buffer) using NBP1-44068 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for COX IV Isoform 2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 17 kDa band observed in human skeletal muscle lysates (calculated MW of 19.6 kDa band according to NP_115998.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COX4-I2

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation. [provided by RefSeq]

Long Name

Cytochrome c Oxidase Subunit IV Isoform 2

Alternate Names

COX4-2, COX4B, COX4L2, COXIV-2

Entrez Gene IDs

84701 (Human); 84682 (Mouse); 84683 (Rat)

Gene Symbol

COX4I2

UniProt

NP_115998.2 (Human)

Additional COX4-I2 Products

Product Documents for COX IV Isoform 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for COX IV Isoform 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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