Cotton Rat IFN-alpha Antibody

Name: Cotton Rat IFN-alpha Antibody
Brand: R&D Systems
SKU: AF1082

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1082

Citations (1)

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Key Product Details

Species Reactivity:	Cotton Rat
Immunogen:	E. coli-derived recombinant cotton rat IFN-alpha Cys24-Lys190 (Cys109Tyr) Accession # AAL18816
Applications:	Block/Neutralize, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects cotton rat IFN-alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human IFN-beta and recombinant cotton rat IFN-gamma is observed.

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Applications for Cotton Rat IFN-alpha Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Cotton Rat IFN-alpha (Cys109Tyr) (Catalog # 1082-CR)

Item	Value
Neutralization	Measured by its ability to neutralize IFN‑ alpha inhibition of EMCV-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND₅₀) is typically 0.015-0.065 µg/mL in the presence of 0.2 ng/mL Recombinant Cotton Rat IFN‑ alpha (Cys109Tyr).

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Cotton Rat IFN-alpha Antibody

	Neutralization IFN‑ alpha Inhibition of EMCV-induced Cytopathy and Neutralization by Cotton Rat IFN‑ alpha Antibody. Recombinant Cotton Rat IFN-a (Cys109Tyr) (Catalog # 1082-CR) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Cotton Rat IFN-a (Cys109Tyr) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1082). The ND₅₀ is typically 0.015-0.065 µg/mL.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IFN-alpha

Interferon-alpha, also known as leukocyte interferon, comprises a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R1) and a 125 kDa subunit (IFN-alpha R2) that is involved both in ligand-binding and signal transduction. IFN-alpha is expressed primarily by leukocytes upon activation by viruses, bacteria, cytokines and growth factors. IFN-alpha has been shown to have anti-viral and immunomodulatory activities on target cells. It can also inhibit cell proliferation as well as induce apoptosis (1, 2).

Cotton rat IFN-alpha 1 cDNA encodes a 189 amino acid (aa) residue precursor protein with a putative 23 aa signal sequence and a 166 aa mature protein. The protein contains five cysteines, four of which are involved in two intrachain disulfide bonds. Cotton rat IFN-alpha 1 shares 52%, 67%, 68% and 72% aa sequence identity to human, rat, hamster and mouse IFN-alpha 1, respectively.

Item	Value
References	Domanski, P. and O.R. Colamonici (1996) Cytokine Growth Factor Rev. 7:143. Pestka, S. (2000) Biopolymers 55:254.
Long Name	Interferon alpha
Entrez Gene IDs	3440 (Human)
Alternate Names	IFN-alpha, IFN-alphaA, IFNA, IFNA2, IFNA2b, IFNalpha, INFA2, LeIF D, interferon alpha 2

Citations for Cotton Rat IFN-alpha Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Cotton Rat IFN-alpha Antibody include:

Species: Cotton Rat
Sample Types: Whole Cells
Applications: Neutralization

Kim D et al. (2013), Synergistic induction of interferon alpha through TLR-3 and TLR-9 agonists identifies CD21 as interferon alpha receptor for the B cell response. PLoS Pathog, 9(3):e1003233.
PMID: 23516365

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