Complement Component C1qA Antibody (JL-1) - Azide Free

Flow Cytometry: Complement Component C1qA Antibody (JL-1) [NBP1-51139]

Flow Cytometry: Complement Component C1qA Antibody (JL-1) [NBP1-51139] - Intracellular flow cytometry for C1q in peritoneal macrophages. Anti-C1q was conjugated to PE using a Lightning Link kit for use in flow cytometry. Macrophages are gated on CD45+CD11b+ and T cells are gated on CD45+CD3+ lymphocytes. Dead cells were excluded, and Fc receptors were blocked to prevent non-specific interactions. This is image was submitted via customer review.

Western Blot: Complement Component C1qA Antibody (JL-1) - Azide Free [NBP1-51139] -

Validation of Nanostring DEGs with Western blot. Tissue samples from the cortex (A) and hippocampus (B) were processed for Western blot analysis to validate the top DEGs derived from Nanostring. A Expression of the DEG markers PSD95 (Dlg4), NCX1 (Slc8a1), Nefl (Neurofilament Light), and PRAS40 (Akt1s1) in cortex tissue. B Expression of the DEG markers GluN2B (Grin2b), C1qA (C1qa), Nefl, and PKC alpha (Prkca) in the hippocampus. n = 6 mice/group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Sham groups, #p < 0.05, ###p < 0.001 vs. SCI/Male. Two-way ANOVA followed by Tukey’s multiple comparison Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37653491), licensed under a CC-BY license. Not internally tested by Novus Biologicals.