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Complement C4b/d Antibody (16D2), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB200-541

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NB200-541

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 16D2

Concentration

0.1 mg/ml

Product Summary for Complement C4b/d Antibody (16D2)

Immunogen

Cell-bound C4 of mouse origin.

Localization

Secreted

Specificity

Antibody cross reacts with C4, C4b and C4d.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Complement C4b/d Antibody (16D2)

Western Blot: Complement C4b/d Antibody (16D2) [NB200-541] - Western blot analysis of C4 expression in J774.A1 (A) aand mouse PBL (B) whole cell lysates.

Applications for Complement C4b/d Antibody (16D2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10

Immunoprecipitation

1:10-1:500

Western Blot

1:10
Application Notes
WB: non-reduced sample treatment and SDS-PAGE was used. IHC-F: Tissue sections were fixed in ice-cold acetone and blocked with 2% BSA, FCS and PBS. Although not confirmed this antibody may be useful in Immunoassays.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 11 publications using NB200-541 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2um filtered PBS and 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Complement C4b/d

In eukaryotic cells, DNA is associated with histones and other proteins to form chromatin. The cell division cycle constitutes a series of processes that have evolved to create two genetically identical daughter cells from a mother cell. One of these processes is the conversion of relatively amorphous, extended interphase chromatin into condensed, highly ordered mitotic chromosomes. Proper mitotic chromosome condensation is essential for the correct segregation of sister chromatids into two daughter cells. The basic unit of chromatin is the nucleosome core particle, which consists of 140 bp DNA wrapped around an octameric core containing two each of the four conserved core histones: H2A, H2B, H3 and H4. A fifth histone, the linker histone H1, interacts with DNA of variable length, linking adjacent nucleosome cores, and further compacting the chromatin. Chromatin changes are initiated during G2 phase of the cell cycle, in preparation for cell division. The most striking morphological change is chromatin condensation, which becomes apparent during prophase and is maximal during the subsequent stages of mitosis. Histone H1 and the N-terminal tail of H3 have key roles in the folding and inter-association of the chromatin fiber. Two currently known phosphorylation sites are present in the N-terminus of H3; serine-10 and serine-28.1,2 Mitogenic stimulation, oncogenic transformation, or induction of oncogenic ras expression are accompanied with increase in serine-10 phosphorylation of the H3 N-terminal domain. Indeed, it has been shown that phosphorylated H3 is associated with c-fos and c-myc genes in stimulated cells. Phosphorylation of H3, at both serine-10 and -28, coincides with the induction of mitotic chromosome condensation. H3 phosphorylation may contribute to proto-oncogene induction by modulating chromatin structure and releasing blocks in elongation. In contrast to H1 hyperphosphorylation, site-specific phosphorylation of core histone H3 at serine-10 and -28 appears to occur exclusively during mitosis in mammalian cells. H3 dephosphorylation occurs quite rapidly after mitosis and serine-10/28 remain unphosphorylated throughout the remainder of interphase. PP1 has been identified as the H3 phosphatase. Monoclonal antibodies reacting specifically with phosphorylated histone H3, are useful tools to study molecular mechanisms associated with the G2 to M transition and chromatin condensation, and for the analysis of protein kinase(s) and phosphatase(s) involved in H3 phosphorylation or dephosphorylation. They may also be used in multiparameter analysis to relate H3 phosphorylation in individual cells to the cell

Alternate Names

basic C4, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3, C4B1, C4B12, C4B2, C4B3, C4FMGC164979, CH, Chido form of C4, CO4C4B5, complement C4-B, complement C4B1a, complement component 4B, complement component 4B (Chido blood group), CPAMD3FLJ60561, EC 2.1.1.144, EC 2.7.11

Entrez Gene IDs

12268 (Mouse)

Gene Symbol

C4B

UniProt

Additional Complement C4b/d Products

Product Documents for Complement C4b/d Antibody (16D2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Complement C4b/d Antibody (16D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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