Collagen IV Antibody - Azide and BSA Free

Immunohistochemistry: Collagen IV Antibody [NBP1-26549]

Collagen-IV-Antibody-Immunohistochemistry-NBP1-26549-img0003.jpg

Immunohistochemistry: Collagen IV Antibody [NBP1-26549] -

Immunohistochemistry: Collagen IV Antibody [NBP1-26549] - Photographs showing H&E staining & immunostaining for Col-I, Col-III, Col-IV & Col-V in the rhesus macaque & baboon intramural tube. Images of open fallopian tube (a, c, e, g, i, k, m, o, q & s) were obtained from control animals. Occluded tube (b, d, f, h, j, l, n, p, r & t) were from animals treated with 5% PF. Arrows indicate fibrotic response. All images were captured at original magnification ×200. Insets (frame r & s) show negative control with an irrelevant antibody. Treatment with PF increased collagen immunoreactivity in the lamina propria of the intramural tube. Image collected & cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S0010782416301445), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Collagen IV Antibody [NBP1-26549] -

Immunohistochemistry: Collagen IV Antibody [NBP1-26549] - Photographs showing H&E staining & immunostaining for Col-I, Col-III, Col-IV & Col-V in the rhesus macaque & baboon intramural tube. Images of open fallopian tube (a, c, e, g, i, k, m, o, q & s) were obtained from control animals. Occluded tube (b, d, f, h, j, l, n, p, r & t) were from animals treated with 5% PF. Arrows indicate fibrotic response. All images were captured at original magnification ×200. Insets (frame r & s) show negative control with an irrelevant antibody. Treatment with PF increased collagen immunoreactivity in the lamina propria of the intramural tube. Image collected & cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S0010782416301445), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen IV Antibody - Azide and BSA Free [NBP1-26549] -

MiR-29a regulation of target gene expression in cochlea from miR-29a+/+ and miR-29a–/– mice. (A–G) With beta-actin as a control, COL4A1, COL4A2, COL4A3, COL4A4, COL4A5, LAMB2, and LAMC1 protein levels in cochlea were analyzed by Western blot. The independent t-test was used to compare data between two groups [COL4A1, t(4) = 9.920, P < 0.001; COL4A2, t(4) = 3.847, P = 0.018; COL4A3, t(4) = 3.389, P = 0.028; COL4A4, t(4) = 2.842, P = 0.047; COL4A5, t(4) = 3.782, P = 0.019; LAMB2, t(4) = 3.915, P = 0.017; LAMC1, t(4) = 3.088, P = 0.037]. (H) The mRNA expression levels of target genes as measured by qRT-PCR [independent t-test, Col4a1, t(4) = 6.050, P = 0.004; Col4a2, t(4) = 13.145, P < 0.001; Col4a3, t(4) = 3.735, P = 0.02; Col4a4, t(4) = 7.238, P = 0.002; Col4a5, t(4) = 13.141, P < 0.001; Lamb2, t(4) = 8.529, P = 0.001; Lamc1, t(4) = 3.126, P = 0.035]. All data are shown as the mean +/- SD, n = 3 biological replicates, *P < 0.05, **P < 0.01, and ***P < 0.001 between two groups. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37275774), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen IV Antibody - Azide and BSA Free [NBP1-26549] -

Nimbidiol treatment normalized the expression of collagen IV, fibronectin and elastin in the diabetic kidney. Representative images from (A) Semi-quantitative RT-PCR analyses showing gene expression and (B) western blot analyses showing protein expression of Col IV, fibronectin and elastin in the kidney. The bar diagrams represent the fold change from WT + Saline. Data are mean +/- SD (n = 6/group). *p < 0.05 versus WT + Saline, WT + Nimbidiol and Akita + Nimbidiol, †p < 0.05 versus Akita + Saline. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36522378), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen IV Antibody - Azide and BSA Free [NBP1-26549] -

Decreased angiogenesis and increased integrin/basement membrane signaling in TNF lungs.(A) Loss of angiogenesis signaling in TNF-Tg mice. MultiNicheNet was used to identify ligand-receptor pairs that were specific to either WT or TNF conditions. WT lungs demonstrated strong Tek, Flt1, Kdr, and Ednrb interactions with Angt1, Vegfa, Gpc3, and Edn3, which were absent TNF-Tg conditions. (B) Increased TGF-beta /integrin/basement membrane signaling in TNF-Tg lungs. TNF-Tg lungs demonstrated the presence of interactions between Itga2 (primarily in gCAP2 cells) and multiple collagen genes, Hspg2, and Tgfb1. (C) Decreased expression of Flt1 and Tek and increased expression of Col4a2 and Itga2 in TNF-Tg gCAP cells. Violin plots demonstrate expression across cell types. (D) Immunostaining confirms increased expression of Col4a1, Itga2, and Igfbp7 in TNF-Tg lungs. Original magnification × 200. (E) Circos plots indicating most differentially regulated ligand-receptor pairs ascertained by MultiNicheNet in WT lungs (left) and TNF-Tg (right) lungs; sender cells are listed on the bottom, while arrows point to receiver cells on the top. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40569693), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen IV Antibody - Azide and BSA Free [NBP1-26549] -

Nimbidiol inhibited upregulation of collagen IV and fibronectin, and downregulation of elastin expression in the diabetic kidney. Representative immunofluorescence images of the renal cortex showing expression of (A) Col IV, (B) fibronectin and (C) elastin. The nuclear counterstaining was performed using DAPI (blue). The bar diagrams represent the fold change in the fluorescence intensity from WT + Saline for (D) Col IV (E) fibronectin and (F) elastin. Data are mean +/- SD (n = 6/group). *p < 0.05 versus WT + Saline, WT + Nimbidiol and Akita + Nimbidiol, †p < 0.05 versus Akita + Saline. Scale bar: 20 um; magnification × 60. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36522378), licensed under a CC-BY license. Not internally tested by Novus Biologicals.