CNF1/CNF2 Antibody (JC4) - Azide Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11249
Key Product Details
Species Reactivity
E. coli
Applications
Western Blot, Immunoassay, Functional
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # JC4
Format
Azide Free
Concentration
0.1 mg/ml
Product Specifications
Immunogen
The monoclonal antibody JC4 is specific for Cytotoxic necrotizing factor type 1 and the highly related Cytotoxic necrotizing factor type 2 (CNF1 and CNF2) of uropathogenic Escherichia coli.
Epitope
Monoclonal antibody JC4 recognizes an epitope between amino acids 169 to 191 of the N-terminal binding domain.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<24 EU/mg
Description
Novus Biologicals Mouse CNF1/CNF2 Antibody (JC4) - Azide Free (NBP3-11249) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Applications for CNF1/CNF2 Antibody (JC4) - Azide Free
Application
Recommended Usage
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunoassay
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CNF1 or CNF2 to be inactivated.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um filtered solution in PBS, 0.1% BSA
Format
Azide Free
Preservative
No Preservative
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CNF1/CNF2
Alternate Names
Cytotoxic necrotizing factor, Cytotoxic necrotizing factor type 1, Cytotoxic necrotizing factor type 2
Additional CNF1/CNF2 Products
Product Documents for CNF1/CNF2 Antibody (JC4) - Azide Free
Product Specific Notices for CNF1/CNF2 Antibody (JC4) - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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