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Claudin-18 Antibody (25E3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26657

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-26657-100ul
NBP3-26657-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # 25E3 expressed in HEK293

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant Human Claudin-18 protein [UniProt P56856]

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

Novus Biologicals Human Claudin-18 Antibody (25E3) - BSA Free (NBP3-26657) is a recombinant monoclonal antibody validated for use in ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Claudin-18 Antibody (25E3) - BSA Free

Claudin-18 Antibody (25E3)

ELISA: Claudin-18 Antibody (25E3) [NBP3-26657] -

ELISA: Claudin-18 Antibody (25E3) [NBP3-26657] - The Binding Activity of Human Claudin-18.2 with Claudin-18 Antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Claudin-18.2 at 5 ug/mL can bind Claudin-18 Antibody, the EC50 is 2.947-8.782 ng/mL.
Claudin-18 Antibody (25E3)

Flow Cytometry: Claudin-18 Antibody (25E3) [NBP3-26657] -

Flow Cytometry: Claudin-18 Antibody (25E3) [NBP3-26657] - Untransfected KATO3 cells (green line), transfected Human CLDN18.2 KATO3 stable cells (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells (blue line) were stained with anti-CLDN18.2 antibody (2ug/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Applications for Claudin-18 Antibody (25E3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% Glycerol, 0.01M PBS, PH 7.4

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Claudin-18

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternate Names

Claudin18, CLDN18, SFTA5, SFTPJ

Gene Symbol

CLDN18

Additional Claudin-18 Products

Product Documents for Claudin-18 Antibody (25E3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Claudin-18 Antibody (25E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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