Claudin-1 Antibody (1F8U5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15397
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1F8U5 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 112-211 of human Claudin-1 (O95832). EVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Claudin-1 Antibody (1F8U5) (NBP3-15397) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Claudin-1 Antibody (1F8U5)
Western Blot: Claudin-1 Antibody (1F8U5) [Claudin-1] -
Western Blot: Claudin-1 Antibody (1F8U5) [Claudin-1] - Western blot analysis of various lysates, using Claudin-1 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Western Blot: Claudin-1 Antibody (1F8U5) [Claudin-1] -
Western Blot: Claudin-1 Antibody (1F8U5) [Claudin-1] - Western blot analysis of various lysates, using Claudin-1 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Applications for Claudin-1 Antibody (1F8U5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Western Blot
1:2000 - 1:12000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Claudin-1
Alternate Names
Claudin1, CLD1, CLDN1, SEMP1
Gene Symbol
CLDN1
Additional Claudin-1 Products
Product Documents for Claudin-1 Antibody (1F8U5)
Product Specific Notices for Claudin-1 Antibody (1F8U5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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