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CKAP2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-21391

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NBP1-21391
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 633 and 683 of human cytoskeleton associated protein 2 using the numbering given in entry NP_001091995.1 (GeneID 26586).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CKAP2 Antibody - BSA Free

Western Blot: CKAP2 Antibody [NBP1-21391]

Western Blot: CKAP2 Antibody [NBP1-21391]

Western Blot: CKAP2 Antibody [NBP1-21391] - Detection of human CKAP2 by western blot. Samples:Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKAP2 antibody NBP1-21391 used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: CKAP2 Antibody [NBP1-21391]

Immunoprecipitation: CKAP2 Antibody [NBP1-21391]

Immunoprecipitation: CKAP2 Antibody [NBP1-21391] - Detection of human CKAP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CKAP2 antibody NBP1-21391 used for IP at 3 ug per reaction. CKAP2 was also immunoprecipitated by rabbit anti-CKAP2 antibody NBP1-21390. For blotting immunoprecipitated CKAP2, NBP1-21391 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for CKAP2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CKAP2

CKAP2 (cytoskeletal-associated protein 2), also known as TMAP (tumor-associated microtubule-associated protein), was identified as an mRNA up-regulated in primary human gastric cancers. It was found to localize to microtubule organizing centers and microtubules and play a role in cell division. CKAP2 is required for proper chromosome segregation and the maintenance of genomic stability. It has recently been shown to be a substrate of Cdk1-cyclinB and essential for bipolar spindle formation.

Alternate Names

CTCL tumor antigen se20-10, cytoskeleton associated protein 2, cytoskeleton-associated protein 2, LB1DKFZp686L1238, se20-10, TMAPFLJ10749, Tumor- and microtubule-associated protein, tumor-associated microtubule-associated protein

Entrez Gene IDs

26586 (Human)

Gene Symbol

CKAP2

Additional CKAP2 Products

Product Documents for CKAP2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CKAP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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