CIRBP Antibody (3V9S7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15745
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3V9S7 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CIRBP (Q14011). MASDEGKLFVGGLSFDTNEQSLEQVFSKYGQISEVVVVKDRETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQAGKSSDNRSRGYRGGSAGGRGFFRGGRGRGRGFSRGGGDRGYGGNRFESRSGGYGGSRDYYSSRSQSGGYSDRSSGGSYRDSYDSYATHNE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CIRBP Antibody (3V9S7) (NBP3-15745) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CIRBP Antibody (3V9S7)
![Western Blot: CIRBP Antibody (3V9S7) [NBP3-15745]](https://resources.bio-techne.com/images/products/CIRBP-Antibody-3V9S7-Western-Blot-NBP3-15745-img0005.jpg "Western Blot: CIRBP Antibody (3V9S7) [NBP3-15745]")
Western Blot: CIRBP Antibody (3V9S7) [NBP3-15745]
Western Blot: CIRBP Antibody (3V9S7) [NBP3-15745] - Analysis of extracts of various cell lines, using CIRBP antibody (NBP3-15745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry: CIRBP Antibody (3V9S7) [NBP3-15745] -
Immunohistochemistry: CIRBP Antibody (3V9S7) [NBP3-15745] - Immunohistochemistry analysis of CIRBP in paraffin-embedded human lung tissue using CIRBP Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunocytochemistry/ Immunofluorescence: CIRBP Antibody (3V9S7) [NBP3-15745] -
Immunocytochemistry/ Immunofluorescence: CIRBP Antibody (3V9S7) [NBP3-15745] - Confocal imaging of paraffin-embedded Human breast cancer tissue using CIRBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.Applications for CIRBP Antibody (3V9S7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CIRBP
Alternate Names
A18 hnRNP, A18HNRNP, CIRPcold-inducible RNA-binding protein, cold inducible RNA binding protein, cold inducible RNA-binding protein, glycine-rich RNA binding protein, Glycine-rich RNA-binding protein CIRP
Gene Symbol
CIRBP
Additional CIRBP Products
Product Documents for CIRBP Antibody (3V9S7)
Product Specific Notices for CIRBP Antibody (3V9S7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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