Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Cip4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human thyroid receptor interacting protein 10 (Cdc42-interacting protein 4) using the numbering given in entry AAM46851.1 (GeneID 9322).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cip4 Antibody - BSA Free

Immunoprecipitation: Cip4 Antibody [NB100-59833]

Immunoprecipitation: Cip4 Antibody [NB100-59833]

Immunoprecipitation: Cip4 Antibody [NB100-59833] - Detection of human TRIP10/CIP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP10/CIP4 antibody NB100-59833 (lot NB100-59833-2) used for IP at 6 ug per reaction. TRIP10/CIP4 was also immunoprecipitated by a previous lot of this antibody (NB100-59833-1) and rabbit anti-TRIP10/CIP4 antibody NB100-59834. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Cip4 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cip4

TRIP10/CIP4 is a multi-functional adapter protein and plays a role in the translocation of Glut4 (glucose transporter) vesicles to the cell surface in response to insulin. TRIP10/CIP4 has also been found to interact with the active GTPases CDC42 and TC10 as well as the Wiskott-Aldrich syndrome protein (WASP), a protein involved in cytoskeletal organization. Recent studies suggest that CIP4 accumulation may play a role in Huntington's disease pathogenesis.

Alternate Names

cdc42-interacting protein 4, CIP4STOT, HSTP, Protein Felic, Salt tolerant protein, salt tolerator, STPTRIP-10, thyroid hormone receptor interactor 10, thyroid receptor interacting protein 10, Thyroid receptor-interacting protein 10, TR-interacting protein 10

Entrez Gene IDs

9322 (Human)

Gene Symbol

TRIP10

UniProt

AAM46851.1 (Human)

Additional Cip4 Products

Product Documents for Cip4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cip4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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