Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33064
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # AH21-10 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within full length human Chorionic Gonadotropin alpha Chain (hCG alpha). (Uniprot: P01215)
Localization
Secreted.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10) (NBP3-33064) is a recombinant monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)
Immunohistochemistry: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10) [NBP3-33064]
Immunohistochemistry: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10) [NBP3-33064] - Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Mouse anti-Chorionic Gonadotropin alpha Chain (hCG alpha) antibody (NBP3-33064) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33064) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:200
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Chorionic Gonadotropin alpha Chain (HCG alpha)
HCG is a glycoprotein hormone secreted in pregnancy by embryonic trophoblast cells during the first 7 weeks of gestation. Production subsequently shifts to the placenta, where it continues until term. HCG is a heterodimer consisting of an alpha chain and a beta chain. The alpha chain is common to several other hormones, including lutenizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the beta chain is unique to HCG. Both alpha and beta chains contain a cysteine knot motif, also found in growth factors such as TGF-beta family members.
Alternate Names
alpha HCG, CGA, FSHA, GPHa1, LHA, TSHA
Gene Symbol
CGA
Additional Chorionic Gonadotropin alpha Chain (HCG alpha) Products
- All Products for Chorionic Gonadotropin alpha Chain (HCG alpha)
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- Chorionic Gonadotropin alpha Chain (HCG alpha) Primary Antibodies
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Product Documents for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)
Product Specific Notices for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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