Chitinase 3-like 1/YKL-40 Antibody (384327) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB25991CL1
![Chitinase 3-like 1/YKL-40 Antibody (384327) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/fab25991cl1_rat-mab-384327-corafluor-1-26920251749413.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Primate
Applications
ELISA Capture (Matched Antibody Pair), Neutralization, In vivo assay
Label
CoraFluor 1
Antibody Source
Monoclonal Rat IgG1 Clone # 384327
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chitinase 3-like 1/YKL-40
Tyr22-Thr383
Accession # P36222
Tyr22-Thr383
Accession # P36222
Specificity
Detects human and primate Chitinase 3-like 1/YKL-40 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for Chitinase 3-like 1/YKL-40 Antibody (384327) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Chitinase 3-like 1/YKL-40 Antibody (384327) [CoraFluor™ 1]
Application
Recommended Usage
ELISA Capture (Matched Antibody Pair)
Optimal dilutions of this antibody should be experimentally determined.
In vivo assay
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Chitinase 3-like 1/YKL-40
Chitinase 3-like 1/YKL-40 (CHI3L1), also called BRP39 in mouse, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.
Alternate Names
CHI3L1, Chitinase 3 like 1, HCgp39, YKL-40
Gene Symbol
CHI3L1
Additional Chitinase 3-like 1/YKL-40 Products
- All Products for Chitinase 3-like 1/YKL-40
- Chitinase 3-like 1/YKL-40 cDNA Clones
- Chitinase 3-like 1/YKL-40 ELISA Kits
- Chitinase 3-like 1/YKL-40 Luminex Assays
- Chitinase 3-like 1/YKL-40 Lysates
- Chitinase 3-like 1/YKL-40 Primary Antibodies
- Chitinase 3-like 1/YKL-40 Proteins and Enzymes
- Chitinase 3-like 1/YKL-40 Simple Plex
Product Documents for Chitinase 3-like 1/YKL-40 Antibody (384327) [CoraFluor™ 1]
Product Specific Notices for Chitinase 3-like 1/YKL-40 Antibody (384327) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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