CHD4 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82651
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (97%), Rat (96%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDDDDDDSKEP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CHD4 Antibody - BSA Free (NBP1-82651) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CHD4 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: CHD4 Antibody [NBP1-82651]](https://resources.bio-techne.com/images/products/CHD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82651-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CHD4 Antibody [NBP1-82651]")
Immunocytochemistry/ Immunofluorescence: CHD4 Antibody [NBP1-82651]
Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP1-82651] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.![Immunohistochemistry-Paraffin: CHD4 Antibody [NBP1-82651]](https://resources.bio-techne.com/images/products/CHD4-Antibody-Immunohistochemistry-Paraffin-NBP1-82651-img0001.jpg "Immunohistochemistry-Paraffin: CHD4 Antibody [NBP1-82651]")
Immunohistochemistry-Paraffin: CHD4 Antibody [NBP1-82651]
Immunohistochemistry-Paraffin: CHD4 Antibody [NBP1-82651] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.![CHD4 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: CHD4 Antibody - BSA Free [NBP1-82651]](https://resources.bio-techne.com/images/products/nbp1-82651_rabbit-polyclonal-chd4-antibody-2562025913983.jpg "Chromatin Immunoprecipitation-exo-Seq: CHD4 Antibody - BSA Free [NBP1-82651]")
Chromatin Immunoprecipitation-exo-Seq: CHD4 Antibody - BSA Free [NBP1-82651]
ChIP-Exo-Seq composite graph for Anti-CHD4 (NBP1-82651) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for CHD4 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CHD4
Alternate Names
ATP-dependent helicase CHD4, CHD-4, chromodomain helicase DNA binding protein 4, chromodomain-helicase-DNA-binding protein 4, DKFZp686E06161, EC 3.6.1, EC 3.6.4.12, Mi-2 autoantigen 218 kDa protein, Mi-2b, Mi2-BETA
Gene Symbol
CHD4
Additional CHD4 Products
Product Documents for CHD4 Antibody - BSA Free
Product Specific Notices for CHD4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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