Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Bovine, Equine

Cited:

Human, Mouse, Bovine, Equine

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all CENPF Antibodies »

Product Specifications

Immunogen

A bacterial fusion protein from the C-terminus of human CENPF. [UniProt# P49454]

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CENPF Antibody - BSA Free

Knockdown Validated: CENPF Antibody - BSA Free [NB500-101]

Immunocytochemistry/ Immunofluorescence: CENPF Antibody - BSA Free [NB500-101]

CENPF-Antibody-Immunohistochemistry-Paraffin-NB500-101-img0009.jpg
Western Blot: CENPF AntibodyBSA Free [NB500-101]

Western Blot: CENPF AntibodyBSA Free [NB500-101]

Western Blot: CENPF Antibody [NB500-101] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.
Immunocytochemistry/ Immunofluorescence: CENPF Antibody - BSA Free [NB500-101]

Immunocytochemistry/ Immunofluorescence: CENPF Antibody - BSA Free [NB500-101]

Immunocytochemistry/Immunofluorescence: CENPF Antibody [NB500-101] - Distribution of CENP-F (red), largely associated with the mitotic spindle and a clearly distinguishable frac.
View 2 more images

Applications for CENPF Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:200-1:1000immunoprecipitation 1:50

Immunohistochemistry Whole-Mount

reported in scientific literature (PMID 24876181)

Immunohistochemistry-Frozen

reported in scientific literature (PMID 26195156)

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 20398247)

Immunoprecipitation

1:50

Western Blot

1:1500-1:2500
Application Notes
In Western Blot, a band can be seen at ~330 kDa. In ICC/IF, nuclear staining was observed in HeLa cells.

Reviewed Applications

Read 2 reviews rated 5 using NB500-101 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CENPF

CENPF interacts with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events.

Alternate Names

AH antigen, cell-cycle-dependent 350K nuclear protein, CENF, CENP-F, CENP-F kinetochore protein, centromere protein F, centromere protein F (350/400kD, mitosin), centromere protein F, 350/400ka (mitosin), centromere protein F, 350/400kDa (mitosin), hcp-1, Kinetochore protein CENPF, mitosin, PRO1779

Gene Symbol

CENPF

Additional CENPF Products

Product Documents for CENPF Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CENPF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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