CENPC Antibody (5E3K5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16305
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5E3K5 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 380-520 of human CENPC (Q03188). VLDTSYALIGETVNNYRSTKYEMYSKNAEKPSRSKRTIKQKQRRKFMAKPAEEQLDVGQSKDENIHTSHITQDEFQRNSDRNMEEHEEMGNDCVSKKQMPPVGSKKSSTRKDKEESKKKRFSSESKNKLVPEEVTSTVTKS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CENPC Antibody (5E3K5) (NBP3-16305) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CENPC Antibody (5E3K5)
![Western Blot: CENPC Antibody (5E3K5) [NBP3-16305]](https://resources.bio-techne.com/images/products/CENPC-Antibody-5E3K5-Western-Blot-NBP3-16305-img0002.jpg "Western Blot: CENPC Antibody (5E3K5) [NBP3-16305]")
Western Blot: CENPC Antibody (5E3K5) [NBP3-16305]
Western Blot: CENPC Antibody (5E3K5) [NBP3-16305] - Western blot analysis of extracts of various cell lines, using CENPC Rabbit mAb (NBP3-16305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: CENPC Antibody (5E3K5) [NBP3-16305] -
Immunocytochemistry/ Immunofluorescence: CENPC Antibody (5E3K5) [NBP3-16305] - Immunofluorescence analysis of C6 cells using CENPC Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for CENPC Antibody (5E3K5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CENPC
Alternate Names
CENP-C, CENP-C 1, CENPCInterphase centromere complex protein 7, Centromere autoantigen C, centromere autoantigen C1, centromere protein C 1, hcp-4, ICEN7, MIF2
Gene Symbol
CENPC
Additional CENPC Products
Product Documents for CENPC Antibody (5E3K5)
Product Specific Notices for CENPC Antibody (5E3K5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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