Cdk9 Antibody (23GB1325)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50258

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NBP3-50258-100ul
NBP3-50258-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1325

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cdk9 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Cdk9 (Uniprot: P50750)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cdk9 Antibody (23GB1325)

Cdk9 Antibody (23GB1325)

Immunocytochemistry/ Immunofluorescence: Cdk9 Antibody (23GB1325)[NBP3-50258] -

Immunocytochemical staining of HepG2 cells with Cdk9 antibody (1:1,000). Nuclei were stained blue with DAPI; Cdk9 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Cdk9 Antibody (23GB1325)

Flow Cytometry: Cdk9 Antibody (23GB1325)[NBP3-50258] -

Flow cytometric analysis of Cdk9expression in HepG2 cells using Cdk9 antibody (1:2,000). Green, isotype control; red, Cyclin dependent kinase 9.
Cdk9 Antibody (23GB1325)

Western Blot: Cdk9 Antibody (23GB1325)[NBP3-50258] -

Western blotting analysis using Cdk9 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit

Applications for Cdk9 Antibody (23GB1325)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cdk9

CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. This kinase also was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. CDK9 (PITALRE) is a ubiquitously expressed nuclear protein.

Long Name

Cyclin-dependent kinase 9

Alternate Names

C-2K, CDC2L4, TAK

Gene Symbol

CDK9

Additional Cdk9 Products

Product Documents for Cdk9 Antibody (23GB1325)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cdk9 Antibody (23GB1325)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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