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CDC2L2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-496

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 730 and the C-terminus (residue 780) of human Cell Division Cycle 2-like 2 using the numbering given in SwissProt entry Q9UQ88 (GeneID 985).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CDC2L2 Antibody - BSA Free (NB100-496) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CDC2L2 Antibody - BSA Free

Western Blot: CDC2L2 Antibody [NB100-496]

Western Blot: CDC2L2 Antibody [NB100-496]

Western Blot: CDC2L2 Antibody [NB100-496] - Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: CDC2L2 Antibody [NB100-496]

Immunohistochemistry-Paraffin: CDC2L2 Antibody [NB100-496]

Immunohistochemistry-Paraffin: CDC2L2 Antibody [NB100-496] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CDK11 (NB100-496). Detection: DAB
Immunoprecipitation: CDC2L2 Antibody [NB100-496]

Immunoprecipitation: CDC2L2 Antibody [NB100-496]

Immunoprecipitation: CDC2L2 Antibody [NB100-496] - Detection of human CDK11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK11 antibody NB100-496 (lot NB100-496-3) used for IP at 3 ug per reaction. CDK11 was also immunoprecipitated by a previous lot of this antibody (lot NB100-496-2). For blotting immunoprecipitated CDK11, NB100-496 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for CDC2L2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CDC2L2

CDK11 is a protein kinase belonging to a large molecular weight protein complex that contains RNA polymerase II. It is associated with the cyclin L regulatory partner and plays a role in influencing transcription and RNA-processing events.

Long Name

Cyclin-dependent kinase 11A

Alternate Names

CDC2L3, CDK11A, PITSLREB

Entrez Gene IDs

728642 (Human)

Gene Symbol

CDK11A

UniProt

Additional CDC2L2 Products

Product Documents for CDC2L2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CDC2L2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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