CD74 Antibody (LN-2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29465
Clone LN-2 was used by HLDA to establish CD designation.
![Flow Cytometry: CD74 Antibody (LN-2) [NBP2-29465]](https://resources.bio-techne.com/images/products/CD74-Antibody-LN-2-Flow-Cytometry-NBP2-29465-img0006.jpg "Flow Cytometry: CD74 Antibody (LN-2) [NBP2-29465]")
![Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465]](https://resources.bio-techne.com/images/products/CD74-Antibody-LN-2-Immunohistochemistry-Paraffin-NBP2-29465-img0004.jpg "Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465]")
![Western Blot: CD74 Antibody (LN-2) [NBP2-29465]](https://resources.bio-techne.com/images/products/CD74-Antibody-LN-2-Western-Blot-NBP2-29465-img0007.jpg "Western Blot: CD74 Antibody (LN-2) [NBP2-29465]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat (Negative)
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # LN-2
Concentration
0.2 mg/ml
Product Specifications
Immunogen
SU-DHL-4 lymphoma cells
Reactivity Notes
Does not react with Rat.
Localization
Cell surface and paranuclear globular
Specificity
It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for CD74 Antibody (LN-2)
Western Blot: CD74 Antibody (LN-2) [NBP2-29465]
Western Blot: CD74 Antibody (LN-2) [NBP2-29465] - Western blot analysis of Raji cell lysate using CD74 antibody (LN-2).![Immunocytochemistry/ Immunofluorescence: CD74 Antibody (LN-2) [NBP2-29465]](https://resources.bio-techne.com/images/products/CD74-Antibody-LN-2-Immunocytochemistry-Immunofluorescence-NBP2-29465-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CD74 Antibody (LN-2) [NBP2-29465]")
Immunocytochemistry/ Immunofluorescence: CD74 Antibody (LN-2) [NBP2-29465]
Immunocytochemistry/Immunofluorescence: CD74 Antibody (LN-2) [NBP2-29465] - Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)![Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465]](https://resources.bio-techne.com/images/products/CD74-Antibody-LN-2-Immunohistochemistry-Paraffin-NBP2-29465-img0005.jpg "Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465]")
Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465]
Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465] - Formalin-fixed, paraffin-embedded human Spleen stained with CD74 Antibody (LN-2).Applications for CD74 Antibody (LN-2)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-4 ug/ml
Immunohistochemistry
0.5-1ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
2-4 ug/ml
Application Notes
Immunofluorescence: (Methanol fixed): 1-4ug/ml.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD74
Additional CD74 Products
Product Documents for CD74 Antibody (LN-2)
Product Specific Notices for CD74 Antibody (LN-2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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