CD45RC Antibody (OX-22) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43542
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # OX-22
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
OX-22 was prepared by immunizing BALB/c mice with phytohemagglutinin-activated rat lymph node cells. (Uniprot# P04157)
Specificity
OX-22 binds specifically to the high molecular weight form of the leucocyte common antigen, CD45RC. OX-22 and OX-31 compete for binding but do not compete with OX-32. The antigen is expresssed on the surface of B cells, around half of bone marrow cells, all CD8+ve T cells, and is able to differentiate between CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like) cell types. CD45 extracellular domain is able to interact with a number of different proteins including CD4, TCR, CD2, Thy-1 and CD26. CD45 is important for signalling throguh the T cell receptor and in the selection of T and B lymphocytes.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Scientific Data Images for CD45RC Antibody (OX-22) - Chimeric - BSA Free
Flow Cytometry: CD45RC Antibody (OX-22) - Chimeric - BSA Free [NBP3-43542] -
Flow Cytometry: CD45RC Antibody (OX-22) - Chimeric - BSA Free [NBP3-43542] - Staining of rat (Rattus norvegicus) lymphocytes with either an isotype control (A) or NBP3-43542 (B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an Alexa Fluor 488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.Applications for CD45RC Antibody (OX-22) - Chimeric - BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD45RC
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
Additional CD45RC Products
Product Documents for CD45RC Antibody (OX-22) - Chimeric - BSA Free
Product Specific Notices for CD45RC Antibody (OX-22) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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