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CD45RB Antibody (PTPRC/2877R), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP3-07180

Recombinant Monoclonal Antibody

Key Product Details

Species Reactivity

Validated:

Human, Rat (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PTPRC/2877R

Concentration

0.2 mg/ml

Product Summary for CD45RB Antibody (PTPRC/2877R)

Immunogen

Recombinant human full-length CD45RB protein (Uniprot: P08575)

Reactivity Notes

Does not react with Rat or Rhesus Monkey.

Localization

Cell Surface

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08924)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for CD45RB Antibody (PTPRC/2877R)

Western Blot: CD45RB Antibody (PTPRC/2877R) [NBP3-07180] - Western Blot Analysis of human spleen cell lysate using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R).
Immunocytochemistry/Immunofluorescence: CD45RB Antibody (PTPRC/2877R) [NBP3-07180] - Paraformaldehyde-fixed Raji cells stained with CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R)followed by Goat anti-rabbit IgG-CF488 (Green). Counterstain is Phalloidin (red).
Immunohistochemistry-Paraffin: CD45RB Antibody (PTPRC/2877R) [NBP3-07180] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R).

Applications for CD45RB Antibody (PTPRC/2877R)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD45RB

CD45RB is an alternatively spliced form of CD45, a cell surface glycoprotein. It is expressed on peripheral B cells, T cytotoxic cells and CD4+ T cells. It has a role in cell signalling regulation through the activation of the Src family of tyrosine kinases.

Long Name

CD45R, B isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

UniProt

Additional CD45RB Products

Product Documents for CD45RB Antibody (PTPRC/2877R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD45RB Antibody (PTPRC/2877R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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