CD36 Antibody (D-2712) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-59724
![Western Blot: CD36 Antibody (D-2712)BSA Free [NB110-59724]](https://resources.bio-techne.com/images/products/CD36-SR-B3-Antibody-D-2712-Western-Blot-NB110-59724-img0007.jpg "Western Blot: CD36 Antibody (D-2712)BSA Free [NB110-59724]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Rat, Human (Negative)
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Proximity Ligation Assay, Westen Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgA Kappa Clone # D-2712
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This CD36 Antibody (D-2712) was developed against adenovirus expressing recombinant mouse CD36
Reactivity Notes
Does not react with human.
Localization
Membrane
Marker
Endothelial Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgA Kappa
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD36 Antibody (D-2712) - BSA Free
Western Blot: CD36 Antibody (D-2712)BSA Free [NB110-59724]
Western Blot: CD36 Antibody (D-2712) [NB110-59724] - Detection of CD36 in mouse adipose lysate.![Immunohistochemistry: CD36 Antibody (D-2712) - BSA Free [NB110-59724]](https://resources.bio-techne.com/images/products/CD36-SR-B3-Antibody-D-2712-Immunohistochemistry-NB110-59724-img0006.jpg "Immunohistochemistry: CD36 Antibody (D-2712) - BSA Free [NB110-59724]")
Immunohistochemistry: CD36 Antibody (D-2712) - BSA Free [NB110-59724]
Immunohistochemistry: CD36 Antibody (D-2712) [NB110-59724] - Staining of CD36 in mouse liver.
Western Blot: CD36 Antibody (D-2712) - BSA Free [NB110-59724] -
Effect of MSCs on cardiac expressions of proteins regulating cholesterol, triglycerides and FFA metabolism. (A) CS exposure caused significant elevation of cardiac expression of LDL-R, which was attenuated in both the BM-MSCs/CS and iPSC-MSCs/CS groups. (B) CS led to increased cardiac expression of HMGCR, which was attenuated in the iPSC-MSCs/CS group. (C) CS reduced cardiac expression of ABCA1, which was restored in the iPSC-MSCs/CS group. (D) CS exposure significantly reduced protein expression of LPL, which was restored in the iPSC-MSCs/CS group. (E) CS exposure led to a significant reduction in protein expression of cardiac CD36, which was significantly restored in the iPSC-MSCs/CS group. (F) Cardiac FAS protein expression was inhibited after CS exposure, which showed a trend of restoration but not reaching significant level in both the BM-MSCs/CS and iPSC-MSCs/CS groups. Cardiac levels of protein expression were normalized to GAPDH levels and expressed as fold change vs. relative control. Data are expressed as mean +/- SEM; n = 7–8. Data were analyzed by one-way ANOVA. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 for comparison between the CS and SA groups. #p < 0.05, ##p < 0.01 for comparison between the BM-MSCs/CS or iPSC-MSCs/CS groups and CS group, respectively. LDL-R, low density lipoprotein receptor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HMGCR, 3-hydroxy-3-methylglutaryl coenzyme A reductase; ABCA1, ATP-binding cassette protein-A1; LPL, Lipoprotein lipase; CD36, fatty acid translocase (FAT)/CD36; FAS, fatty acid synthase. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28804458), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for CD36 Antibody (D-2712) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:250
Immunohistochemistry-Paraffin
1:100-1:250
Immunoprecipitation
10-20 ug
Western Blot
1:500
Application Notes
In Western blot, a band is observed at ~70-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW, as seen in Western blot, is most likely due to the heavy glycosylation and palmitoylation of this protein.
Reviewed Applications
Read 1 review rated 3 using NB110-59724 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD36
The expression of CD36 has been reported in platelets, erythrocytes, monocytes, differentiated adipocytes, skeletal muscle, mammary epithelial cells, spleen cells, some skin microdermal endothelial cells, and in cancer. Circulating levels of soluble CD36 (sCD36) has also been reported in chronic inflammatory disease such as type 2 diabetes and chronic kidney disease. CD36 participates in angiogenesis, innate immunity, and the clearance of apoptotic phagocytes. In lipid metabolism, CD36 functions as a macrophage receptor for oxidized LDL and as an adipocyte receptor/transporter for long-chain FFAs. Plasmodium falciparum, the parasite that causes malaria, binds CD36 via PfEMP1 proteins, tethering parasite-infected erythrocytes to endothelial receptors (5). Anti-CD36 isoantibodies have been detected in Type 1 CD36-deficient mothers and is implicated as the cause of fetal/neonatal alloimmune thrombocytopenia (6).
References
1) Febbraio, M., Hajjar, D. P., & Silverstein, R. L. (2001). CD36: a class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. The Journal of clinical investigation, 108(6), 785-791. PMID: 11560944
2) Silverstein RL, Febbraio M. (2000) CD36 and atherosclerosis. Curr Opin Lipidol. 2000 11(5):483-91. PMID: 11048891.
3) Endemann G, Stanton LW, Madden KS, Bryant CM, White RT, Protter AA. (1993) CD36 is a receptor for oxidized low density lipoprotein. J Biol Chem. 268(16):11811-6. PMID: 7685021.
4) Wang, J., & Li, Y. (2019). CD36 tango in cancer: signaling pathways and functions. Theranostics, 9(17), 4893-4908. PMID: 31410189
5) Hsieh FL, Turner L, Bolla JR, Robinson CV, Lavstsen T, Higgins MK. (2016) The structural basis for CD36 binding by the malaria parasite. Nat Commun. 7:12837. PMID: 27667267
6) Gruarin P, Ulliers D, Thorne RF, Alessio M. (2000) Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb. Mol Cell Biochem 214, 115-121. PMID: 11195795.
Alternate Names
BDPLT10, CD36 antigen (collagen type I receptor, thrombospondin receptor), CD36 molecule (thrombospondin receptor), CHDS7, cluster determinant 36, FAT, Fatty acid translocase, Glycoprotein IIIb, GP3B, GP4, GPIIIB, GPIV, leukocyte differentiation antigen CD36, PAS IV, PAS-4 protein, PASIV, platelet glycoprotein 4, platelet glycoprotein IV, SCARB3, scavenger receptor class B, member 3
Gene Symbol
CD36
Additional CD36 Products
Product Documents for CD36 Antibody (D-2712) - BSA Free
Product Specific Notices for CD36 Antibody (D-2712) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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