CD3 gamma Antibody (7J2L5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16327
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7J2L5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 83-182 of human CD3 gamma (P09693). GMYQCKGSQNKSKPLQVYYRMCQNCIELNAATISGFLFAEIVSIFVLAVGVYFIAGQDGVRQSRASDKQTLLPNDQLYQPLKDREDDQYSHLQGNQLRRN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD3 gamma Antibody (7J2L5)
![Western Blot: CD3 gamma Antibody (7J2L5) [NBP3-16327]](https://resources.bio-techne.com/images/products/CD3-gamma-Antibody-7J2L5-Western-Blot-NBP3-16327-img0002.jpg "Western Blot: CD3 gamma Antibody (7J2L5) [NBP3-16327]")
Western Blot: CD3 gamma Antibody (7J2L5) [NBP3-16327]
Western Blot: CD3 gamma Antibody (7J2L5) [NBP3-16327] - Western blot analysis of extracts of various cell lines, using CD3 gamma Rabbit mAb (NBP3-16327) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
Immunocytochemistry/ Immunofluorescence: CD3 gamma Antibody (7J2L5) [NBP3-16327] -
Immunocytochemistry/ Immunofluorescence: CD3 gamma Antibody (7J2L5) [NBP3-16327] - Immunofluorescence analysis of Jurkat cells using CD3 gamma Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for CD3 gamma Antibody (7J2L5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CD3 gamma
Alternate Names
CD3g, IMD17, T3G
Gene Symbol
CD3G
Additional CD3 gamma Products
Product Documents for CD3 gamma Antibody (7J2L5)
Product Specific Notices for CD3 gamma Antibody (7J2L5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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