CD25/IL-2R alpha Antibody (OX-39) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-64761
![Immunocytochemistry/ Immunofluorescence: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]](https://resources.bio-techne.com/images/products/CD25-IL-2-R-alpha-Antibody-OX-39-Immunocytochemistry-Immunofluorescence-NB100-64761-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]")
![Immunocytochemistry/ Immunofluorescence: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]](https://resources.bio-techne.com/images/products/CD25-IL-2-R-alpha-Antibody-OX-39-Immunocytochemistry-Immunofluorescence-NB100-64761-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, ELISA, Flow Cytometry, Immunoprecipitation
Cited:
Immunohistochemistry-Frozen, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OX-39
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Stimulated Rat T cells
Localization
Cell Membrane
Specificity
NB100-64761 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) ^(6). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD25/IL-2R alpha Antibody (OX-39) - BSA Free
Immunocytochemistry/ Immunofluorescence: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]
Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power![Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]](https://resources.bio-techne.com/images/products/CD25-IL-2-R-alpha-Antibody-OX-39-Immunohistochemistry-Paraffin-NB100-64761-img0002.jpg "Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]")
Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]
Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power![Flow Cytometry: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]](https://resources.bio-techne.com/images/products/CD25-IL-2-R-alpha-Antibody-OX-39-Flow-Cytometry-NB100-64761-img0003.jpg "Flow Cytometry: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]")
Flow Cytometry: CD25/IL-2R alpha Antibody (OX-39) - BSA Free [NB100-64761]
Flow Cytometry: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: BiotinApplications for CD25/IL-2R alpha Antibody (OX-39) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD25/IL-2R alpha
Long Name
Interleukin 2 Receptor alpha
Alternate Names
CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA
Entrez Gene IDs
25704 (Rat)
Gene Symbol
IL2RA
UniProt
Additional CD25/IL-2R alpha Products
Product Documents for CD25/IL-2R alpha Antibody (OX-39) - BSA Free
Product Specific Notices for CD25/IL-2R alpha Antibody (OX-39) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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