CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12071

Submit a review

Product Datasheet / COA

Recombinant Monoclonal Antibody

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP3-12071AF350

Alexa Fluor 405

NBP3-12071AF405

Alexa Fluor 488

NBP3-12071AF488

Alexa Fluor 532

NBP3-12071AF532

Alexa Fluor 594

NBP3-12071AF594

Alexa Fluor 647

NBP3-12071AF647

Alexa Fluor 700

NBP3-12071AF700

Alexa Fluor 750

NBP3-12071AF750

Allophycocyanin

NBP3-12071APC

Biotin

NBP3-12071B

CoraFluor 1

NBP3-12071CL1

DyLight 350

NBP3-12071UV

DyLight 405

NBP3-12071V

DyLight 488

NBP3-12071G

DyLight 550

NBP3-12071R

DyLight 594

NBP3-12071DL594

DyLight 650

NBP3-12071C

DyLight 680

NBP3-12071FR

DyLight 755

NBP3-12071IR

FITC

NBP3-12071F

HRP

NBP3-12071H

Janelia Fluor 525

NBP3-12071JF525

Janelia Fluor 549

NBP3-12071JF549

Janelia Fluor 646

NBP3-12071JF646

Janelia Fluor 669

NBP3-12071JF669

mFluor Violet 450 SE

NBP3-12071MFV450

mFluor Violet 500 SE

NBP3-12071MFV500

mFluor Violet 610 SE

NBP3-12071MFV610

PE

NBP3-12071PE

PerCP

NBP3-12071PCP

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # CBT6

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD1a Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunizing mice with the human CD1a protein.

Specificity

The antibody is specific for the human CD1a protein.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Novus Biologicals Rabbit CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free (NBP3-12071) is a recombinant monoclonal antibody validated for use in IHC, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free [NBP3-12071]

Immunocytochemistry/Immunofluorescence: CD1a Antibody (CBT6) - Chimeric [NBP3-12071] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of CBT6 (NBP3-12071) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12071, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free [NBP3-12071] -

Flow Cytometry: CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free [NBP3-12071] - Molt4 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12071 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD1a

All CD1 molecules, except CD1e, are cell surface glycoproteins that are structurally related to the MHC molecules, however, in distinction, CD1 proteins are essentially non polymorphic. CD1 has considerable structural homology with both MHC class I and class II molecules, and CD1 molecules are involved in T cell activation. In contrast to MHC, however, CD1 molecules appear to present predominantly non peptide molecules originating from lipids and glycolipids.

Long Name

T Cell Surface Glycoprotein CD1a

Alternate Names

CD1a, FCB6, HTA1, R4, T6

Gene Symbol

CD1A

Additional CD1a Products

Product Documents for CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for CD1a Antibody (CBT6) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews