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CD161/NK1.1 Antibody (PK136) - BSA Free

Catalog # NB100-77528 | Novus Biologicals a Bio-Techne Brand
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NB100-77528
NB100-77528SS

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # PK136

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for CD161/NK1.1 Antibody (PK136) - BSA Free

Immunogen

NK-1+ cells from mouse spleen and bone marrow

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD161/NK1.1 Antibody (PK136) - BSA Free

Flow Cytometry: CD161/NK1.1 Antibody (PK136) [NB100-77528] - Analysis using the PE/Cy7 conjugate of NB100-77528. Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC and staining buffer (autofluorescence) (left) or Anti-Mouse NK1.1 PE-Cy7 (right). Total viable cells were used for analysis.
Flow Cytometry: CD161/NK1.1 Antibody (PK136) [NB100-77528] - Mouse splenocytes were stained with CD49b (DX5) APC and purified NK1.1 (clone PK136) (top) or mouse IgG2a isotype control (bottom), followed by anti-mouse IgG2a FITC.
Flow Cytometry: CD161/NK1.1 Antibody (PK136) [NB100-77528] - C57BL/6 mouse splenocytes stained with purified PK136, followed by anti-mouse IgG2a FITC

Applications for CD161/NK1.1 Antibody (PK136) - BSA Free

Application
Recommended Usage

Flow Cytometry

< 1.0 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

1:20-1:1000

Immunohistochemistry

1:20-1:1000

Immunohistochemistry-Frozen

1:20-1:1000

Immunohistochemistry-Paraffin

1:20-1:1000. Use reported in scientific literature (PMID 21118981)

Immunoprecipitation

1:20-1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 0.25 ug/1 x10^6 cells in 100 ul. It is recommended that the reagent be titrated for optimal performance for each application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 10 publications using NB100-77528 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD161/NK1.1

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Alternate Names

Klrb1c, Ly-59, Ly55c

Entrez Gene IDs

17057 (Mouse)

Gene Symbol

Klrb1c

Product Documents for CD161/NK1.1 Antibody (PK136) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD161/NK1.1 Antibody (PK136) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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