Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

CCR5 Antibody (12D1)

Catalog # NBP2-44483 | Novus Biologicals a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-44483-0.02mg
NBP2-44483-0.1mg

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 12D1

Concentration

0.2 mg/ml

Product Summary for CCR5 Antibody (12D1)

Immunogen

Human native CCR5 protein (Uniprot: P51681)

Localization

Cell surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47759)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for CCR5 Antibody (12D1)

Immunocytochemistry/Immunofluorescence: CCR5 Antibody (12D1) [NBP2-44483] - Immunofluorescence staining of PFA-fixed HeLa cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-mouse IgG-CF488 (green).
Immunohistochemistry-Paraffin: CCR5 Antibody (12D1) [NBP2-44483] - Human Stomach tissue stained with CCR5 Antibody (12D1).
Flow Cytometry: CCR5 Antibody (12D1) [NBP2-44483] - Flow Cytometric Analysis of MCF-7 cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for CCR5 Antibody (12D1)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Frozen

1-2 ug/ml
Application Notes
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CCR5

C-C chemokine receptor type 5 (CCR5) is a member of the beta chemokine receptors family of integral membrane proteins. The CCR5 protein is a G protein-coupled receptor which functions as a chemokine receptor in the CC chemokine group.

CCR5 is a major coreceptor for human HIV infection and is predominantly expressed on T cells, macrophages, dendritic cells and microglia. It is likely that CCR5 plays a role in inflammatory responses to infection, though its exact role in normal immune function is unclear.

CCR5 antibodies are useful for HIV infection studies and research on immune responses.

Alternate Names

CCR5, CD195

Gene Symbol

CCR5

UniProt

Additional CCR5 Products

Product Documents for CCR5 Antibody (12D1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCR5 Antibody (12D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...