CCR1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56334

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NB100-56334
NB100-56334SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rabbit

Cited:

Human, Mouse, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular)

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Flow (Cell Surface), Flow - IC, Flow Ctyometry, Flow Cytometry Control

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all CCR1 Antibodies »

Product Specifications

Immunogen

This antibody was raised against a synthetic peptide of human CCR1 protein.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 20371596).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCR1 Antibody - BSA Free

Western Blot: CCR1 Antibody [NB100-56334]

Western Blot: CCR1 Antibody [NB100-56334]

Western Blot: CCR1 Antibody [NB100-56334] - A375 lysate probed with this antibody at 2 ug/ml dilution.
Flow (Intracellular): CCR1 Antibody [NB100-56334]

Flow (Intracellular): CCR1 Antibody [NB100-56334]

Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on Raw264.7 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
Flow Cytometry: CCR1 Antibody [NB100-56334]

Flow Cytometry: CCR1 Antibody [NB100-56334]

Flow Cytometry: CCR1 Antibody [NB100-56334] - Image 1: Surface staining of mouse primary epidermal cells (B-6 mice, 30wks) with CL 11-PE, CD11c PE-Cy5, and this antibody with CY5 goat anti rabbit secondary. Image 2: CD11c+ CL II+ cells (Langerans), stained with rabbit isotype control (shaded histogram) were analyzed for CCR1 at 5 ul (blue histogram) and 10 ul (green histogram). Data kindly provided by Corey Parlet, University of Iowa.
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Applications for CCR1 Antibody - BSA Free

Application
Recommended Usage

Flow (Intracellular)

reported in scientific literature (PMID 20371596)

Flow Cytometry

5-10 ul/test

Immunohistochemistry

1:200

Immunohistochemistry-Frozen

reported in scientific literature (PMID 15860797)

Immunohistochemistry-Paraffin

1:200

Western Blot

1-3 ug/ml
Application Notes
In A375, a 42 kDa band should be observed.

Reviewed Applications

Read 1 review rated 4 using NB100-56334 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCR1

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR1, is expressed on neutrophils, monocytes, lymphocytes, and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1), eotaxin, as well as several other related chemokines (2-4). Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation (5,6).

Alternate Names

CCR1, CD191

Entrez Gene IDs

1230 (Human); 12768 (Mouse)

Gene Symbol

CCR1

UniProt

NP_001286.1 (Human)

Additional CCR1 Products

Product Documents for CCR1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CCR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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