Key Product Details

Species Reactivity

Mouse

Applications

ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)

Label

Janelia Fluor 525

Antibody Source

Monoclonal Rat IgG1 Clone # 53433

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CCL5/RANTES Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL5/RANTES
Ser24-Ser91
Accession # P30882

Specificity

Detects recombinant mouse (rm)CCL5 direct ELISAs. In sandwich ELISAs, approximately 5% cross-reactivity with rhCCL5 and less than 0.2% cross-reactivity with rhTECK and rhVIC was observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for CCL5/RANTES Antibody (53433) [Janelia Fluor® 525]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CCL5/RANTES

RANTES (Regulated-on-activation-normal-T-cell-expressed-and-secreted) is a member of the "intercrine/chemokine" family of cytokines, has been previously shown to be chemotactic for monocytes and T cells of memory/helper phenotype. RANTES has been shown to cause the release of histamine from basophils and activates eosinophils. It also binds to CCR1, CCR3, CCR4 and CCR5 (1). This CC-chemokine enhances antigen-specific T helper (Th) type-1 responses against HIV-1 (2). CC chemokines are small inducible proteins that are related to interleukin 8. In lung adenocarcinoma, RANTES is a predictor of survival while that of interleukin (IL)-8 is associated with a poor prognosis. In several models, tumorigenesis is abolished by RANTES, while it is facilitated by IL-8. Site-directed mutagenesis indicated that regulation of RANTES promoter activity required two nuclear factor (NF)-kappaB response elements but not its activator protein (AP)-1 binding sites (3).

Alternate Names

RANTES, SISd

Gene Symbol

CCL5

Additional CCL5/RANTES Products

Product Documents for CCL5/RANTES Antibody (53433) [Janelia Fluor® 525]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CCL5/RANTES Antibody (53433) [Janelia Fluor® 525]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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