CCL4/MIP-1 beta Antibody [Dylight 550]
Novus Biologicals, part of Bio-Techne | Catalog # AF6935L
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Western Blot, Neutralization
Label
DyLight 550 (Excitation = 562 nm, Emission = 576 nm)
Antibody Source
Polyclonal Sheep IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
E. coli-derived recombinant rat CCL4/MIP-1 beta
Ala24-Asn92
Accession # P50230
Ala24-Asn92
Accession # P50230
Specificity
Detects rat CCL4/MIP-1 beta in direct ELISAs and Western blots. In direct ELISAs, approximately 8% cross-reactivity with recombinant mouse CCL4/MIP-1 beta and recombinant rat CCL3/MIP-1 alpha is observed, and less than 2% cross-reactivity with recombinant human CCL4/MIP-1 beta is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for CCL4/MIP-1 beta Antibody [Dylight 550]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product AF6935. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CCL4/MIP-1 beta
CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor.
Alternate Names
Exodus-3, MIP-1 beta, MIP1 beta
Gene Symbol
CCL4
Additional CCL4/MIP-1 beta Products
Product Documents for CCL4/MIP-1 beta Antibody [Dylight 550]
Product Specific Notices for CCL4/MIP-1 beta Antibody [Dylight 550]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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