CCL2/JE/MCP-1 Antibody (280707) [DyLight 650]
Novus Biologicals, part of Bio-Techne | Catalog # FAB2817W
Conjugate
Catalog #
Key Product Details
Species Reactivity
Canine
Applications
Western Blot
Label
DyLight 650 (Excitation = 652 nm, Emission = 672 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 280707
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
E. coli-derived recombinant canine CCL2/JE/MCP-1
Gln24-Pro101 (predicted)
Accession # P52203
Gln24-Pro101 (predicted)
Accession # P52203
Specificity
Detects canine CCL2/JE/MCP‑1 in direct ELISAs and Western blots. In direct ELISAs, 80-100% cross-reactivity with recombinant human CCL2 and recombinant mouse SDF-1 alpha is observed. In Western blots, no cross-reactivity with recombinant human
CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,
26, 27, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22, 24, 25,
27, or recombinant rat CCL20 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for CCL2/JE/MCP-1 Antibody (280707) [DyLight 650]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CCL2/JE/MCP-1
CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
Additional CCL2/JE/MCP-1 Products
Product Documents for CCL2/JE/MCP-1 Antibody (280707) [DyLight 650]
Product Specific Notices for CCL2/JE/MCP-1 Antibody (280707) [DyLight 650]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...