CCAR1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-188

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all CCAR1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide, mapping to a region region between residue 1100 and the C-terminus (residue 1150) of human Cell-Cycle and Apoptosis Regulatory Protein 1 using the numbering given in TrEMBL entry Q6X935 (GeneID 55749).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CCAR1 Antibody - BSA Free (NB500-188) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CCAR1 Antibody - BSA Free

Western Blot: CCAR1 Antibody [NB500-188]

Western Blot: CCAR1 Antibody [NB500-188]

Western Blot: CCAR1 Antibody [NB500-188] - Detection of Human CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-188 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: CCAR1 Antibody [NB500-188]

Western Blot: CCAR1 Antibody [NB500-188]

Western Blot: CCAR1 Antibody [NB500-188] - on HeLa whole cell lysate using NB500-188. CCAR1 was also immunoprecipitated with another rabbit anti-CCAR1 antibody, NB500-187, and NB500-188. Immunoprecipitated CCAR1 was blotted using NB500-188.

Applications for CCAR1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-10 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
NB 500-188 may be used for Western blot and immunoprecipitation. Suggested working dilutions: * Western Blot: 1:1,000 - 1:10,000 Immunoprecipitation: 1 to 10 ug/mg lysate Histo/Cytochemistry: not tested. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CCAR1

DIS is thought to be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Therefore DIS would have a role in cell cycle progression and/or cell proliferation.

Alternate Names

CARP-1, CARP1MGC44628, Cell cycle and apoptosis regulatory protein 1, cell division cycle and apoptosis regulator 1, cell division cycle and apoptosis regulator protein 1, Death inducer with SAP domain, DIS, FLJ10590, RP11-437A18.1

Entrez Gene IDs

55749 (Human)

Gene Symbol

CCAR1

UniProt

Q6X935 (Human)

Additional CCAR1 Products

Product Documents for CCAR1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CCAR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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