Caspase-7 Antibody (25B881.1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56529
![Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]](https://resources.bio-techne.com/images/products/Caspase-7-Antibody-25B881-1-Western-Blot-NB100-56529-img0008.jpg "Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 25B881.1
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was generated against recombinant protein of active form of human Caspase-7.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Caspase-7 Antibody (25B881.1) - BSA Free
Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]
Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.![Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]](https://resources.bio-techne.com/images/products/Caspase-7-Antibody-25B881-1-Western-Blot-NB100-56529-img0009.jpg "Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]")
Western Blot: Caspase-7 Antibody (25B881.1)BSA Free [NB100-56529]
Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.![Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) - BSA Free [NB100-56529]](https://resources.bio-techne.com/images/products/Caspase-7-Antibody-25B881-1-Immunohistochemistry-Paraffin-NB100-56529-img0007.jpg "Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) - BSA Free [NB100-56529]")
Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) - BSA Free [NB100-56529]
Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) [NB100-56529] - Human tonsil stained with Caspase-7 antibody at 5 ug/ml. Novus' human tonsil tissue slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Applications for Caspase-7 Antibody (25B881.1) - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-2 ug/ml
Application Notes
In Jurkat, a 35 kDa band is observed.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Caspase-7
Additional Caspase-7 Products
Product Documents for Caspase-7 Antibody (25B881.1) - BSA Free
Product Specific Notices for Caspase-7 Antibody (25B881.1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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