Caspase-7 Antibody (23GB2310)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50254
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB2310
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human pro Caspase-7 (Uniprot: P55210)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Caspase-7 Antibody (23GB2310)
Immunocytochemistry/ Immunofluorescence: Caspase-7 Antibody (23GB2310)[NBP3-50254] -
Immunocytochemical staining of HepG2 cells with Caspase-7 antibody (1:1,000). Nuclei were stained blue with DAPI; Caspase 7 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: Caspase-7 Antibody (23GB2310)[NBP3-50254] -
Flow cytometric analysis of Caspase-7expression in HepG2 cells using Caspase-7 antibody (1:2,000). Green, isotype control; red, Caspase 7.
Western Blot: Caspase-7 Antibody (23GB2310)[NBP3-50254] -
Western blotting analysis using Caspase-7 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for Caspase-7 Antibody (23GB2310)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Caspase-7
Caspase 7 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 7 form.
Caspase 7 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.
Alternate Names
CASP7, Caspase7, Mch3
Gene Symbol
CASP7
Additional Caspase-7 Products
Product Documents for Caspase-7 Antibody (23GB2310)
Product Specific Notices for Caspase-7 Antibody (23GB2310)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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