Caspase-6 Antibody (23GB5635)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51363

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NBP3-51363-100ul
NBP3-51363-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB5635

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Caspase-6 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Caspase-6 (Uniprot: P55212)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caspase-6 Antibody (23GB5635)

Caspase-6 Antibody (23GB5635)

Immunocytochemistry/ Immunofluorescence: Caspase-6 Antibody (23GB5635)[NBP3-51363] -

Immunocytochemical staining of C2C12 cells with Caspase-6 antibody (1:1,000). Nuclei were stained blue with DAPI; Caspase-6 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
Caspase-6 Antibody (23GB5635)

Western Blot: Caspase-6 Antibody (23GB5635)[NBP3-51363] -

Western blotting analysis using Caspase-6 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for Caspase-6 Antibody (23GB5635)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-6

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).

Alternate Names

CASP6, Caspase6, Mch2

Gene Symbol

CASP6

Additional Caspase-6 Products

Product Documents for Caspase-6 Antibody (23GB5635)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caspase-6 Antibody (23GB5635)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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