Casein Kinase 2 alpha Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-378
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-378]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-378-img0012.jpg "Western Blot: Casein Kinase 2 alpha Antibody [NB100-378]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Bovine (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 350 and the C-terminus (residue 391) of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Casein Kinase 2 alpha Antibody - BSA Free
Western Blot: Casein Kinase 2 alpha Antibody [NB100-378]
Western Blot: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-378]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-NB100-378-img0010.jpg "Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-378]")
Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-378]
Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-378] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB![Immunoprecipitation: Casein Kinase 2 alpha Antibody [NB100-378]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Immunoprecipitation-NB100-378-img0013.jpg "Immunoprecipitation: Casein Kinase 2 alpha Antibody [NB100-378]")
Immunoprecipitation: Casein Kinase 2 alpha Antibody [NB100-378]
Immunoprecipitation: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of human CKII alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 (lot NB100-378-2) used for IP at 6 ug/mg lysate. CKII alpha was also immunoprecipitated by a previous lot (lot NB100-378-1) of this antibody. For blotting immunoprecipitated CKII alpha, NB100-378 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for Casein Kinase 2 alpha Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:2000 - 1:10000
Immunohistochemistry-Paraffin
1:2000 - 1:10000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000- 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Casein Kinase 2 alpha
Additional Casein Kinase 2 alpha Products
Product Documents for Casein Kinase 2 alpha Antibody - BSA Free
Product Specific Notices for Casein Kinase 2 alpha Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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