Carboxylesterase 1/CES1 Antibody (7C9R6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15388
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7C9R6 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Carboxylesterase 1/CES1 (P23141). MWLRAFILATLSASAAWGHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSEL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Carboxylesterase 1/CES1 Antibody (7C9R6)
![Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388]](https://resources.bio-techne.com/images/products/Carboxylesterase-1-CES1-Antibody-7C9R6-Western-Blot-NBP3-15388-img0002.jpg "Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388]")
Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388]
Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388] - Western blot analysis of extracts of various cell lines, using Carboxylesterase 1/CES1 Rabbit mAb (NBP3-15388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunocytochemistry/ Immunofluorescence: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388]](https://resources.bio-techne.com/images/products/Carboxylesterase-1-CES1-Antibody-7C9R6-Immunocytochemistry-Immunofluorescence-NBP3-15388-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388]")
Immunocytochemistry/ Immunofluorescence: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388]
Immunocytochemistry/Immunofluorescence: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388] - Immunofluorescence analysis of HeLa cells using Carboxylesterase 1/CES1 Rabbit mAb (NBP3-15388) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388]](https://resources.bio-techne.com/images/products/Carboxylesterase-1-CES1-Antibody-7C9R6-Western-Blot-NBP3-15388-img0003.jpg "Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388]")
Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388]
Western Blot: Carboxylesterase 1/CES1 Antibody (7C9R6) [NBP3-15388] - Western blot analysis of extracts of HepG2 cells, using Carboxylesterase 1/CES1 Rabbit mAb (NBP3-15388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Applications for Carboxylesterase 1/CES1 Antibody (7C9R6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Carboxylesterase 1/CES1
Carboxylesterase 1 hydrolyzes ester- and amide bonds of substrates ranging from small molecule esters such as phenylester to long-chain fatty acid esters and thioesters. It plays a major role in the pharmacokinetic behavior of many therapeutic agents containing an ester. Through de-esterification, it participates in the detoxification of drugs such as cocaine and heroin in serum and liver.
Alternate Names
ACAT, CES1, Egasyn, HMSE1, REH, SES1, TGH
Gene Symbol
CES1
Additional Carboxylesterase 1/CES1 Products
Product Documents for Carboxylesterase 1/CES1 Antibody (7C9R6)
Product Specific Notices for Carboxylesterase 1/CES1 Antibody (7C9R6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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