Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Janelia Fluor 525

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Carbohydrate Sulfotransferase 1/CHST1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 1/CHST1
Arg24-Ser411
Accession # O43916

Specificity

Detects human Carbohydrate Sulfotransferase 1/CHST1 in direct ELISAs and Western blots. In Western blots, approximately 50% cross‑reactivity with recombinant mouse (rm) CHST1 is observed and less than 5% cross‑reactivity with recombinant human (rh) CHST2, rhCHST5, and rmCHST7 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Carbohydrate Sulfotransferase 1/CHST1

The CHST family is comprised of 14 enzymes in humans. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids. This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses. CHST1, also known as keratan sulfate Gal-6 sulfotransferase, transfers sulfate to position 6 of galactose residues on keratan sulfate. It also has sulfotransferase activity on sialyl N-acetyllactosamine structures and participates in biosynthesis of selectin ligands that play a central role in lymphocyte homing at sites of inflammation. Human CHST1 shares 94% amino acid sequence identity with mouse CHST1.

Alternate Names

6-O-sulfotransferase 1, CHST1, GST-1, KS6ST, KSGal6ST, KSST

Gene Symbol

CHST1

Additional Carbohydrate Sulfotransferase 1/CHST1 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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