Canine IL-5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1964
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Canine
Applications
Immunocytochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant canine IL-5
Phe20-Ser134
Accession # Q95J76
Phe20-Ser134
Accession # Q95J76
Specificity
Detects canine canine IL‑5 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Canine IL-5 Antibody
IL‑5 in Canine PBMCs.
IL-5 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Canine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1964) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cell Proliferation Induced by IL-5 and Neutralization by Canine IL-5 Antibody.
Recombinant Canine IL-5 (1964-CL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-5 (30 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Canine IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1964). The ND50 is typically 0.75-3.0 µg/mL.Applications for Canine IL-5 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA
Sample: Immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA
Western Blot
0.1 µg/mL
Sample: Recombinant Canine IL-5 (Catalog # 1964-CL)
Sample: Recombinant Canine IL-5 (Catalog # 1964-CL)
Neutralization
Measured by its ability to neutralize IL-5-induced proliferation in the TF-1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 30 ng/mL Recombinant Canine IL-5.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5
Interleukin-5 (IL-5) is a 40‑45 kDa secreted disulfide-linked homodimeric glycoprotein that plays an important role in the differentiation, growth, and function of eosinophils. It also primes basophils for histamine and leukotriene release. In mice, IL-5 also induces the proliferation, differentiation, and immunoglobulin production of B cells especially B-1 cells that constitutively express IL-5 receptor alpha. IL-5 is primarily produced by CD4+ Th2 cells. Other cell types, including mast cells, visceral smooth muscle cells, bronchial epithelium, CD16+ NK cells, eosinophils and gamma delta T cells, can also produce IL-5. Canine IL-5 is synthesized as a 134 amino acid (aa) precursor that contains a 21 aa signal sequence and a 113 aa mature segment. Mature canine IL-5 shares 62%, 66%, 85%, 84%, 58%, and 56% aa sequence identity with mature human, guinea pig, porcine, feline, mouse, and rat IL-5, respectively. The receptor for IL-5 consists of a 60 kDa ligand-binding subunit (IL-5 R alpha) and a 120 kDa signal-transducing subunit ( betac) (1‑7).
References
- Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
- Karlen, S. et al. (1998) Intern. Rev. Immunol. 16:227.
- Takatsu, K. (1998) Cytokine Growth Factor Rev. 9:25.
- Zabeau, L. et al. (2003) Curr. Drug Targets Inflamm. Allergy 2:319.
- Yang, S. et al. (2001) J. Interferon Cytokine Res. 21:361.
- Jans, D.A. and G. Hassan (1998) BioEssays 20:400.
- Geijsen, N. et al. (2001) Cytokine Growth Factor Rev. 12:19.
Long Name
Interleukin 5
Alternate Names
BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF
Entrez Gene IDs
Gene Symbol
IL5
UniProt
Additional IL-5 Products
Product Documents for Canine IL-5 Antibody
Product Specific Notices for Canine IL-5 Antibody
For research use only
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