CaM Kinase II [p Thr286] Antibody (JE51-81)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32752
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE51-81 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr286 of human CaM Kinase II. (Uniprot: Q13554)
Modification
p Thr 286
Localization
Synapse, Postsynaptic density, Cell projection, dendritic spine, dendrite.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit CaM Kinase II [p Thr286] Antibody (JE51-81) (NBP3-32752) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CaM Kinase II [p Thr286] Antibody (JE51-81)
![CaM Kinase II [p Thr286] Antibody (JE51-81)](https://resources.bio-techne.com/images/products/nbp3-32752_rabbit-cam-kinase-ii-p-thr286-mab-je51-81-288202412461682.jpg "Western Blot: CaM Kinase II [p Thr286] Antibody (JE51-81) [NBP3-32752]")
Western Blot: CaM Kinase II [p Thr286] Antibody (JE51-81) [NBP3-32752]
Western Blot: CaM Kinase II [p Thr286] Antibody (JE51-81) [NBP3-32752] - Western blot analysis of CaM Kinase II on different lysates with Rabbit anti-CaM Kinase II antibody (NBP3-32752) at 1/1,000 dilution.Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Mouse brain treated with pp for 1 hour tissue lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 54~73 kDa
Observed band size: 50~60 kDa
Exposure time: 53 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for CaM Kinase II [p Thr286] Antibody (JE51-81)
Application
Recommended Usage
Western Blot
1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CaM Kinase II
Calmodulin kinase II (CaMKII) is an 8 - 12 subunit multimeric calcium/calmodulin dependent Ser/Thr protein kinase. It is ubiquitously expressed and interacts with a very diverse group of substrates. In rat, there are four possible subunits/isozymes (alpha, beta, gamma, delta), each of which contains a catalytic, autoregulatory, and subunit-association domain.
Long Name
Calcium/Calmodulin-dependent Protein Kinase II
Alternate Names
CAMK2, CAMK2B
Gene Symbol
CAMK2B
Additional CaM Kinase II Products
Product Documents for CaM Kinase II [p Thr286] Antibody (JE51-81)
Product Specific Notices for CaM Kinase II [p Thr286] Antibody (JE51-81)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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