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CaM Kinase II alpha Antibody (6G9), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB100-1983

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Xenopus

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Radioimmunoassay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6G9

Concentration

1 mg/ml

Product Summary for CaM Kinase II alpha Antibody (6G9)

Immunogen

Partially purified rat CaMKII

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28421537). Xenopus reactivity reported in scientific literature (PMID: 29412139). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasm , Mitochondrion , Nucleus , Cell Junction , Synapse , Presynaptic Cell Membrane

Specificity

Detects approx 50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CaM Kinase II alpha Antibody (6G9)

Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Western Blot analysis of Rat brain membrane lysate showing detection of CaM Kinase II alpha protein using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:1000.
Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.
Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunohistochemistry analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.

Applications for CaM Kinase II alpha Antibody (6G9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:2000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:10000
Application Notes
0.1 ug/ml was sufficient for detection of CamKII in 20 ug rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 14 publications using NB100-1983 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaM Kinase II alpha

CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

Long Name

Calcium/Calmodulin-dependent Protein Kinase II alpha

Alternate Names

CAMK2A

Entrez Gene IDs

815 (Human); 12322 (Mouse); 25400 (Rat)

Gene Symbol

CAMK2A

UniProt

Additional CaM Kinase II alpha Products

Product Documents for CaM Kinase II alpha Antibody (6G9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CaM Kinase II alpha Antibody (6G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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